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KMID : 0613820100200060934
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Journal of Life Science
2010 Volume.20 No. 6 p.934 ~ p.939
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Improvement of Antibacterial Activities of Bacteriocidal Yeasts Using the GPD Promoter
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Jang Min-Kyung
Lee Dong-Geun
Yu Ki-Hwan
Kim Nam-Young
Lee Ok-Hee
Shin Jae-Kyun
Jang Hye-Ji
Lee Seung-Woo
Lee Sang-Hyeon
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Abstract
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We have previously reported recombinant productions of bacteriocins using yeast expression plasmid pAUR123, which contains the alcohol dehydrogenase (ADH) promoter, in Saccharomyces cerevisiae cells and their antibacterial activities. In order to improve the antibacterial activities of bacteriocidal yeast cells, a strong glyceraldehyde phosphate dehydrogenase (GPD) promoter gene of S. cerevisiae was amplified and inserted upstream into bacteriocin genes such as the OR-7, Subpeptin JM4-A or JM4-B gene in the corresponding recombinant yeast plasmid. Yeast cells transformed by the recombinant plasmid containing the GPD promoter represented higher antibacterial activities against both Gram positive B. subtilis and Gram negative E. coli cells compared to those transformed by the corresponding recombinant plasmid containing the ADH promoter. Thus, yeast cells harboring the recombinant plasmid containing the GPD promoter constructed in this study could be applied in the food preservative or animal feed industries.
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KEYWORD
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Bacteriocidal yeast, bacteriocin, GPD promoter, OR-7, Subpeptin JM4-A, Subpeptin JM4-B
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